Tion and have been performed simultaneously on each tissue set. Most of
These constructs were introduced through Agrobacterium tumefasciens-mediated vacuum infiltration into apetala1-15 mutant plants (Columbia ecotype;Gocal et al.Bechtold et al., 1993). Transformants were selected on 0.five Murashige and Skoog plates containing 50 g mL 1 kanamycin. Inflorescences have been fixed and examined utilizing scanning electron microscopy as described ([142. Till these days the presence of a functioning T3SS has been] Weigel et al., 1992).ACKNOWLEDGMENTS The authors thank Drs. Improvement 119: 721?43 Bradley D, Carpenter R, Copsey L, Vincent C, Rothstein S, Coen E (1996) Handle PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20569196 of inflorescence Nal extension of AtTIM17-2 apparently doesn't include mitochondrial targeting architecture in Antirrhinum. Nature 379: 791?97 Busch MA, Bomblies K, Weigel D (1999) Activation of a floral homeotic gene in Arabidopsis. Science 285: 585?87 Carpenter R, Coen ES (1990) Floral homeotic mutations created by transposon mutagenesis in Antirrhinum majus. Genes Dev four: 1483?493 Carpenter R, Copsey L, Vincent C, Doyle S, Magrath R, Coen E (1995) Handle of flower development and phyllotaxy by meristem identity genes in Antirrhinum. Plant Cell 7: 2001?011 Chandler PM, Higgins TJV, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25486018 Randall PJ, Spencer D (1983) Regulation of legumin levels in establishing pea seeds below situations of sulfur deficiency. Plant Physiol 71: 47?Coen ES, Meyerowitz EM (1991) The war with the whorls: genetic interactions controlling flower improvement. Nature 353: 31?7.Tion and had been performed simultaneously on each tissue set. The majority of the in situ outcomes presented had been from material collected in a single experiment (Lt434). T-DNA Constructs cDNAs of LtMADS1, LtMADS2, and its alternatively spliced version LtMADS2 , have been cloned among a 1.7-kb fragment of the Arabidopsis APETALA1 promoter upstream in the ATG (HindIII/EcoICR I; Hempel et al., 1997) and also the pea (Pisum sativum) RBCSE9 terminator (accession no. M21375) within a pBluescriptKS (Stratagene) derivative termed pGG62. Each and every pAP1:LtMADS:RBCSE9 construct was shuttled as a HindIII fragment into pCGN1547 (McBride and Summerfelt, 1990). These constructs had been introduced via Agrobacterium tumefasciens-mediated vacuum infiltration into apetala1-15 mutant plants (Columbia ecotype;Gocal et al.Bechtold et al., 1993). Transformants had been chosen on 0.five Murashige and Skoog plates containing 50 g mL 1 kanamycin. No less than 20 T1 lines had been obtained per construct and amongst four and six were selected randomly for subsequent evaluation. For LtMADS1 and LtMADS2 , floral organs were counted for the initial four to eight flowers and for LtMADS2 all flowers have been counted (up to 4 per plant). Inflorescences were fixed and examined employing scanning electron microscopy as described (Weigel et al., 1992).ACKNOWLEDGMENTS The authors thank Drs. Liz Dennis, Lloyd Evans, Frank Gubler, Masumi Robertson (CSIRO, Plant Market, Canberra) and Malcolm Whitecross (Australian National University, Canberra) for their assistance and valuable comments. Greg Gocal can also be indebted to a number of scientists in the Australian National University, the Commonwealth Scientific and Industrial Investigation Organization, and the plant molecular biology community in San Diego. Received November 20, 2000; returned for revision December 18, 2000; accepted January 10, 2001.LITERATURE CITED Ambrose BA, Lerner DR, Ciceri P, Padilla CM, Yanofsky MF, Schmidt RJ (2000) Molecular and genetic evaluation of your Silky1 gene reveals conservation in floral organ specification between eudicots and monocots.