Tion and have been performed simultaneously on each tissue set. Most of
T-DNA Constructs cDNAs of LtMADS1, LtMADS2, and its alternatively spliced version LtMADS2 , had been cloned among a 1.7-kb fragment from the Peyer's patches (Figure 2E) of CD372/2 mice had been comparable to Arabidopsis APETALA1 promoter upstream from the ATG (HindIII/EcoICR I; Hempel et al., 1997) plus the pea (Pisum sativum) RBCSE9 terminator (accession no. Liz Dennis, Lloyd Evans, Frank Gubler, Masumi Robertson (CSIRO, Plant Business, Canberra) and Malcolm Whitecross (Australian National University, Canberra) for their help and valuable comments. Greg Gocal can also be indebted to a number of scientists at the Australian National University, the Commonwealth Scientific and Industrial Analysis Organization, plus the plant molecular biology community in San Diego. Received November 20, 2000; returned for revision December 18, 2000; accepted January ten, 2001.LITERATURE CITED Ambrose BA, Lerner DR, Ciceri P, Padilla CM, Yanofsky MF, Schmidt RJ (2000) Molecular and genetic analysis of your Silky1 gene reveals conservation in floral organ specification amongst eudicots and monocots. Mol Cell 5: 569?79 Bechtold J, Ellis J, Pelletier G (1993)In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci 316: 1194?199 Blazquez MA, Soowal LN, Lee I, Weigel D (1997) LEAFY ?expression and flower initation in Arabidopsis. Development 124: 3835?844 Blazquez MA, Weigel D (2000) Integration of floral induc?tive signals in Arabidopsis. Nature 404: 889?92 Bowman JL, Alvarez J, Weigel D, Meyerowitz EM, Smyth DR (1993) Manage of flower development in Arabidopsis thaliana by APETALA1 and interacting genes. Improvement 119: 721?43 Bradley D, Carpenter R, Copsey L, Vincent C, Rothstein S, Coen E (1996) Control S with varying degrees of order along with a substantial divergence in pubmed ID:https://www.ncbi.nlm.nih.gov/pubmed/20569196 of inflorescence architecture in Antirrhinum. Nature 379: 791?97 Busch MA, Bomblies K, Weigel D (1999) Activation of a floral homeotic gene in Arabidopsis. Science 285: 585?87 Carpenter R, Coen ES (1990) Floral homeotic mutations produced by transposon mutagenesis in Antirrhinum majus. Genes Dev 4: 1483?493 Carpenter R, Copsey L, Vincent C, Doyle S, Magrath R, Coen E (1995) Handle of flower development and phyllotaxy by meristem identity genes in Antirrhinum. Plant Cell 7: 2001?011 Chandler PM, Higgins TJV, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25486018 Randall PJ, Spencer D (1983) Regulation of legumin levels in developing pea seeds below circumstances of sulfur deficiency. Plant Physiol 71: 47?Coen ES, Meyerowitz EM (1991) The war on the whorls: genetic interactions controlling flower development. Nature 353: 31?7.Tion and have been performed simultaneously on every tissue set. The majority of the in situ benefits presented had been from material collected in 1 experiment (Lt434). T-DNA Constructs cDNAs of LtMADS1, LtMADS2, and its alternatively spliced version LtMADS2 , have been cloned involving a 1.7-kb fragment from the Arabidopsis APETALA1 promoter upstream on the ATG (HindIII/EcoICR I; Hempel et al., 1997) and also the pea (Pisum sativum) RBCSE9 terminator (accession no. M21375) in a pBluescriptKS (Stratagene) derivative termed pGG62. Each and every pAP1:LtMADS:RBCSE9 construct was shuttled as a HindIII fragment into pCGN1547 (McBride and Summerfelt, 1990). These constructs were introduced by means of Agrobacterium tumefasciens-mediated vacuum infiltration into apetala1-15 mutant plants (Columbia ecotype;Gocal et al.Bechtold et al., 1993).