Gradation stimulated by EGF.Expression of EGFR in gastric cancer correlates

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c SGC-7901 cells were transfected with control siRNA or siRhoA, and the expressions of RhoA and EGFR 2-Hydroxy-4-methoxybenzaldehydePurity & Documentation pubmed ID:https://www.ncbi.nlm.nih.gov/pubmed/27906190 have been measured. Overall, the clinical data support our in vitro results that CD24 may play a role as an EGFR supporter to promote gastric cancer progression.Discussion Aberrant activation CD24 signaling contributes to malignant progression of gastric cancer has been demonstrated recently [4, 17]. Here, we investigated the relationship between CD24 and EGFR in gastric cancer cells. We demonstrated here that knockdown of CD24 accelerates internalization and PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26240184 decreases membranous stability of EGFR, top to an impaired EGFR signaling cascade and cell migration. In addition, we foundthat CD24-mediated activation of RhoA is essential for maintaining EGFR expression. Additionally, evaluation of gastric cancer specimens also showed a optimistic correlation between CD24 and EGFR expression.Gradation stimulated by EGF.Expression of EGFR in gastric cancer correlates with CD24 expressionTo investigate whether or not our experimental findings may be relevant towards the pathogenesis of gastric cancer inDeng et al. J Transl Med (2016) 14:Page 7 ofFig. three Downregulation of CD24 alters the localization and degradation of EGFR. a The mRNA levels of CD24 and EGFR were detected by qPCR in SGC-7901 cells transfected with handle siRNA or siCD24. b and c The amount of EGFR on cellular membrane of SGC-7901 cells (b) and BGC-823 cells (c) was measured making use of flow cytometry. d Representative immunofluorescence photos of SGC-7901 cells transfected with control siRNA or siCD24 staining for EGFR and phalliodin. Scale bar 10 M. Asterisk P PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27906190 had been measured. EGFR bands had been quantified and normalized against GAPDH (graph around the correct). Asterisk P